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Imaging super-risoluzione per studiare la co-localizzazione di proteine e marcatori sinaptici nei neuroni primari
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Neurociência
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JoVE Journal Neurociência
Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
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Imaging super-risoluzione per studiare la co-localizzazione di proteine e marcatori sinaptici nei neuroni primari

5,610 Views

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14:02 min

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October 31, 2020

DOI:

10.3791/61434-v

DOI:
10.3791/61434-v

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14:02 min
October 31, 2020

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1Department of Molecular Biochemistry and Pharmacology,Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 2Department of Neuroscience,Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 3Dulbecco Telethon Institute,Istituto di Ricerche Farmacologiche Mario Negri IRCCS

Tags

Super-resolution ImagingCo-localizationPrimary NeuronsSynaptic MarkersStructured Illumination Microscopy (SIM)Neurodegenerative DisordersHippocampal NeuronsFixationImmunostainingMounting SolutionCoverglass

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Russo, L., Natale, C., Conz, A., Kelk, J., Restelli, E., Chiesa, R., Salmona, M., Fioriti, L., Colnaghi, L. Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons. J. Vis. Exp. (164), e61434, doi:10.3791/61434 (2020).

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