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Mätning av nukleotidbindning till intakta, funktionella membranproteiner i realtid
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
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Mätning av nukleotidbindning till intakta, funktionella membranproteiner i realtid

1,614 Views

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08:33 min

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March 11, 2021

DOI:

10.3791/61401-v

DOI:
10.3791/61401-v

•

08:33 min
March 11, 2021

•

3 Views
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1Department of Physiology, Anatomy and Genetics,University of Oxford, 2Department of Chemistry and Neuroscience Program,Trinity College

Tags

Nucleotide BindingMembrane ProteinsFRETATPPotassium ChannelDiabetesUnroofed MembraneSonicationMicroscopyANAPFluorescence Imaging

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Usher, S. G., Ashcroft, F. M., Puljung, M. C. Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time. J. Vis. Exp. (169), e61401, doi:10.3791/61401 (2021).

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