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用于植物微RNA检测和定量的RNA斑点分析
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JoVE Journal Bioquímica
RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
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用于植物微RNA检测和定量的RNA斑点分析

DOI:
10.3791/61394-v

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14:41 min

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July 11, 2020

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, ,
1National Centre for Biological Sciences,Tata Institute of Fundamental Research, 2SASTRA University

Capítulos

  • 00:00Introduction
  • 01:27Step 1: Preparation of a 15% Denaturing Polyacrylamide Gel
  • 02:03Step 2: Assembly of the Glass Plates and Electrophresis Unit
  • 04:13Step 3: Preparation of Loading Dye and Samples
  • 05:38Step 4: Gel Electrophresis
  • 06:33Step 5: Preparation for Electro-blotting
  • 10:15Step 6: Preparation of Radiolabeled Probe
  • 10:50Step 7: Hybridization of the Blot
  • 12:36Representative Results
  • 13:24Conclusion

Summary

Tadução automática

Tadução automática

此方法演示了利用北方杂交技术从总RNA提取物中检测miRNA。

Tags

RNA Blot AnalysisMicroRNA DetectionSmall RNA Northern BlotAcrylamide Gel ElectrophoresisRNA QuantificationPrecursor MicroRNA DetectionModified Northern Protocol

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