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RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

JoVE Journal
Bioquímica
Author Produced

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JoVE Journal Bioquímica

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

DOI:

10.3791/61394-v

•

14:41 min

•

July 11, 2020

•

Varsha Tirumalai², Melvin Prasad, P. V. Shivaprasad

¹National Centre for Biological Sciences,Tata Institute of Fundamental Research, ²SASTRA University

Capítulos

00:00Introduction
01:27Step 1: Preparation of a 15% Denaturing Polyacrylamide Gel
02:03Step 2: Assembly of the Glass Plates and Electrophresis Unit
04:13Step 3: Preparation of Loading Dye and Samples
05:38Step 4: Gel Electrophresis
06:33Step 5: Preparation for Electro-blotting
10:15Step 6: Preparation of Radiolabeled Probe
10:50Step 7: Hybridization of the Blot
12:36Representative Results
13:24Conclusion

Summary

Tadução automática

This method demonstrates use of the northern hybridization technique to detect miRNAs from total RNA extract.

Tags

RNA Blot Analysis MicroRNA Detection Small RNA Northern Blot Acrylamide Gel Electrophoresis RNA Quantification Precursor MicroRNA Detection Modified Northern Protocol

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