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Chemistry

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JoVE Journal Chemistry
Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
 

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

Article DOI: 10.3791/61342-v • 09:24 min • December 18th, 2020
December 18th, 2020 •
1, 1,2,3,4
1Department of Chemistry, Vanderbilt University, 2Vanderbilt Memory & Alzheimer's Center, Vanderbilt University Medical Center, 3Vanderbilt Brain Institute, Vanderbilt University Medical Center, 4Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center

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Combined precursor isotopic labeling and isobaric tagging (cPILOT) is an enhanced sample multiplexing strategy that is capable of increasing the number of samples that can be analyzed simultaneously with available isobaric tags. Incorporation of a robotic platform has greatly increased experimental throughput, reproducibility, and quantitative accuracy.

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Keywords: Automated CPILOT High Throughput Biological Information Aging Alzheimer's Disease Liver Homogenate Urea Denaturation Buffer DTT IAM Cystine
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