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Journal / Chemistry / A Quantitative Glycomics and Proteomics Combined…
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Chemistry

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JoVE Journal Chemistry
A Quantitative Glycomics and Proteomics Combined Purification Strategy
 

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Article DOI: 10.3791/53735-v • 11:38 min • March 8th, 2016
March 8th, 2016 •
*1, *1, 1
1Department of Chemistry, North Carolina State University
* These authors contributed equally

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A high-throughput protocol was developed for combined proteomics and glycomics purification and LC-MS/MS quantification in plasma. Deamidation analysis of N-linked glycosylation motifs was specific to deglycosylated sites. Accurate quantitation of N-glycans was achieved by coupling filter aided N-glycan separation to the individuality normalization when labeling with glycan hydrazide tags strategy.

Tags

Quantitative Glycomics Proteomics Purification Strategy N-glycans Proteins Mass Spectrometry Analysis Scalability Speed Analytical Reproducibility Glycomics Glycosylation Site Occupancy High-throughput Biological Studies N-glycan Measurements Mass Spectrometry Tailored Derivatization Strategy Relative Quantification Cancer And Control States Analytical Variability Biological Interactions Glycome Proteome Disease States Biomarker Discovery
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