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通过异位细胞系统中的蛋白质聚合测量跨细胞相互作用
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Neurociência
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JoVE Journal Neurociência
Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System
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通过异位细胞系统中的蛋白质聚合测量跨细胞相互作用

DOI:
10.3791/61237-v

•

04:47 min

•

May 22, 2020

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, , ,
1Department of Pharmacology,University of Colorado Denver School of Medicine

Capítulos

  • 00:04Introduction
  • 00:31Cell Harvesting
  • 02:12Image Acquisition
  • 02:50Results: Point Mutation Enhances Binding
  • 04:15Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们提出了一个优化的协议，使用荧光显微镜快速和半定量测量异质细胞系统中跨体中的配体受体相互作用。

Tags

Protein AggregationTranscellular InteractionsCell-based AssayTrans AdhesionHeterologous Cell SystemHEK-293T CellsEDTAHemocytometerFluorescence ImagingProtein Of InterestLigand Of InterestSynaptic Ligand

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