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Imunologia e Infecção

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斑马鱼幼虫感染 阿斯珀吉卢斯 孢子分析宿主-病原体相互作用

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions

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斑马鱼幼虫感染阿斯珀吉卢斯孢子分析宿主-病原体相互作用

DOI:

10.3791/61165-v

•

09:42 min

•

May 16, 2020

•

Savini Thrikawala, Emily E. Rosowski

¹Department of Biological Sciences,Clemson University

Capítulos

00:04Introduction
00:57Zebrafish Larva Hindbrain Ventricle Microinjection
04:21Establishment of Injected and Viable Spore Numbers
05:22Drug Treatment of Larvae and Survival Analysis
05:53Zebrafish Wounding and Entrapment Device for Growth and Imaging
07:23Results: Infection Outcome of Aspergillus Spores Microinjected into Zebrafish Larvae
09:07Conclusion

Summary

Tadução automática

该协议描述了斑马鱼幼虫的 阿斯珀吉勒斯 感染模型。孢子被微注入幼虫的后脑，化学处理用于诱导免疫抑制。感染进展通过每日成像设置进行监测，以监测真菌生长和免疫反应，以及通过菌落形成单位电镀来列举活孢子。

Tags

Zebrafish Larvae Aspergillus Infection Host-pathogen Interactions Microinjection Pressure Injector Aspergillus Fumigatus Opportunistic Pathogen Invasive Aspergillosis Immune Response Therapeutic Targets

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