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Immunology and Infection

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JoVE Journal Immunology and Infection
Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions
 

Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions

Article DOI: 10.3791/61165-v • 09:42 min • May 16th, 2020
May 16th, 2020 •
1, 1
1Department of Biological Sciences, Clemson University

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This protocol describes an Aspergillus infection model in zebrafish larvae. Aspergillus spores are microinjected into the hindbrain of larvae, and chemical treatment is used to induce immunosuppression. Infection progression is monitored via a daily imaging setup to monitor fungal growth and immune responses as well as enumeration of live spores by colony forming unit plating.

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Zebrafish Larvae Aspergillus Infection Host-pathogen Interactions Immune Responses Larval Zebrafish Model Therapeutic Targets Invasive Aspergillosis Microinjections Pressure Injector Vac Pressure Unit Foot Switch Micropipette Holder Micro Manipulator Magnetic Stand And Plate Compressed Air Valve Microinjector PBS Spore Suspension Phenol Red Aspergillus Fumigatus
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