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Klickchemischer fluorometrischer Assay für Apolipoprotein-N-Acyltransferase von der Enzymcharakterisierung bis zum Hochdurchsatz-Screening
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Bioquímica
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JoVE Journal Bioquímica
Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
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Klickchemischer fluorometrischer Assay für Apolipoprotein-N-Acyltransferase von der Enzymcharakterisierung bis zum Hochdurchsatz-Screening

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07:37 min

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May 13, 2020

DOI:

10.3791/61146-v

DOI:
10.3791/61146-v

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07:37 min
May 13, 2020

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1Institut Pasteur, Unité Biologie et génétique de la paroi bactérienne, Paris, France ; CNRS, UMR 2001 « Microbiologie intégrative et Moléculaire », Paris, France ; INSERM, Équipe Avenir, Paris, France

Tags

Click-chemistryFluorometric AssayApolipoprotein N-acyltransferaseEnzyme CharacterizationHigh-throughput ScreeningIntegral Membrane EnzymeLipidDetergentSonicationAlkyne POPEFSL-1-biotinLNT Reaction BufferStreptavidinAzido-FAMTCEPTBTACopper SulfatePBST1Nonhomogeneous SuspensionPotential Antibiotics

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Nozeret, K., Pernin, A., Buddelmeijer, N. Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening. J. Vis. Exp. (159), e61146, doi:10.3791/61146 (2020).

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