The Flypub assay measures the behaviors that the fruit fly Drosophila melanogaster displays under the influence of ethanol. The assay can be readily mastered by experimenters at all levels and applied to various vaporized stimuli, facilitating substance abuse and addiction studies.
Delgado, N. M., Sierra, C. M., Arzola, A., Saldes, E. B., Han, K., Sabandal, P. R. Flypub To Study Ethanol Induced Behavioral Disinhibition and Sensitization. J. Vis. Exp. (159), e61123, doi:10.3791/61123 (2020).