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Investigation of Xenobiotics Metabolism In Salix alba Leaves via Mass Spectrometry Imaging
 

Investigation of Xenobiotics Metabolism In Salix alba Leaves via Mass Spectrometry Imaging

Article DOI: 10.3791/61011-v • 09:21 min • June 15th, 2020
June 15th, 2020 •
1, 1,2, 1
1Plant Imaging and Mass Spectrometry (PIMS), Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 2Département mécanique, ICube Laboratoire des sciences de l'ingénieur, de l'informatique et de l'imagerie, UNISTRA/CNRS/ENGEES/INSA

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This method uses mass spectrometry imaging (MSI) to understand metabolic processes in S. alba leaves when exposed to xenobiotics. The method allows the spatial localization of compounds of interest and their predicted metabolites within specific, intact tissues.

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Xenobiotics Metabolism Salix Alba Leaves Mass Spectrometry Imaging Metabolite Identification Tissue Localization Sample Preparation Compound Extraction Cryo Microtome Embedding Medium Cutting Thickness Temperature Adjustment Glass Microscope Slide Cryo Microtome Chamber
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