Journal
/
Neurociência
/
通过宫内注射诱导利普托门宁皮细胞修饰
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Induction of Leptomeningeal Cells Modification Via Intracisternal Injection
Please note that all translations are automatically generated. Click here for the English version.

通过宫内注射诱导利普托门宁皮细胞修饰

DOI:
10.3791/61009-v

•

05:55 min

•

May 07, 2020

•

, ,
1Department of Cell and Molecular Biology,Karolinska Institute

Capítulos

  • 00:04Introduction
  • 01:07Preparation of the Injection System
  • 02:54Intracisternal Injection
  • 03:55Results: Specific Labelling of Leptomeningeal Cells via Intracisternal Injection of Endoxifen
  • 05:07Conclusion

Summary

Tadução automática

Tadução automática

我们描述了一种宫内注射，在头尖弯曲的针头可以稳定在头骨上，从而消除了对底层腹腔损伤的风险。该方法可用于脑膜蛋白酶细胞的基因命运图谱和操作，以及跟踪脑脊液运动。

Tags

Keywords: Leptomeningeal CellsIntracisternal InjectionGene EditingNeurodevelopmentBacterial MeningitisCerebrospinal Fluid FlowHamilton SyringeEndoxifenIsofluraneCisterna MagnaDural MembraneSurgical Procedure

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/61009?article_type=v

在完整的感觉器官前体细胞的活细胞成像果蝇蛹
check_url/pt/61009?article_type=v

人类神经祖细胞和神经元小分子诱导人类胚胎干细胞的多能干高效的推导
check_url/pt/61009?article_type=v

扩展胚胎及成体神经干细胞<em>在子宫内</em电穿孔或病毒立体定向注射
check_url/pt/61009?article_type=v

设计，包装，并通过立体定向注射高效价CRISPR复古和慢交付
check_url/pt/61009?article_type=v

用于访问视网膜下腔的另类和验证注射法<i>通过</i>一经巩膜后路
check_url/pt/61009?article_type=v

在Vivo直接重新编程居民胶质细胞到内神经通过病毒载体的脑内注射
check_url/pt/61009?article_type=v

使用动脉内血压监测和通气通过气管切除术的小鼠脑死亡诱导
check_url/pt/61009?article_type=v

成人皮层的器官型培养作为人类干细胞移植和验证的 离体 模型
check_url/pt/61009?article_type=v

软脑膜淋巴内皮细胞的收获和原代培养
check_url/pt/61009?article_type=v

通过跨内皮电阻检测对bEnd.3血管内皮细胞进行屏障功能完整性记录

Read Article