É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Center for Biological Systems Analysis,University of Freiburg, 2Faculty of Biology,University of Freiburg, 3Freiburg Institute for Advanced Studies (FRIAS),University of Freiburg, 4BIOSS Centre for Biological Signalling Studies,University of Freiburg, 5IMTEK Department of Microsystems Engineering,University of Freiburg, 6Cluster of Excellence livMatS at FIT – Freiburg Center for Interactive Materials and Bioinspired Technologies,University of Freiburg
Capítulos
- 00:05Introduction
- 00:45Protein Expression and Preparation
- 03:38Dye-modification of Elastin-like Proteins via SPAAC
- 04:44THF Swelling Protocol
- 06:29BuOH Extrusion Protocol
- 07:19Results: THF Swelling Method and the BuOH Extrusion Method for the Guided Self-assembly of Amphiphilic ELPs into Supramolecular Structures
- 09:22Conclusion
På grænsefladen af organiske og vandige opløsningsmidler samles skræddersyede amfifile elastin-lignende proteiner i komplekse supramolekylære strukturer såsom vesikler, fibre og coacervater udløst af miljømæssige parametre. De beskrevne montageprotokoller giver Protein Membrane-Based Compartments (PMBC' er) med tunable-egenskaber, hvilket muliggør indkapsling af forskellige laster.
