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Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
 

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Article DOI: 10.3791/60935-v • 10:01 min • April 8th, 2020
April 8th, 2020 •
*1,2, *1,2, 1,2, 1,2, 1,2,3,4,5,6
1Center for Biological Systems Analysis, University of Freiburg, 2Faculty of Biology, University of Freiburg, 3Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, 4BIOSS Centre for Biological Signalling Studies, University of Freiburg, 5IMTEK Department of Microsystems Engineering, University of Freiburg, 6Cluster of Excellence livMatS at FIT - Freiburg Center for Interactive Materials and Bioinspired Technologies, University of Freiburg
* These authors contributed equally

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At the interface of organic and aqueous solvents, tailored amphiphilic elastin-like proteins assemble into complex supramolecular structures such as vesicles, fibers and coacervates triggered by environmental parameters. The described assembly protocols yield Protein Membrane-Based Compartments (PMBCs) with tunable properties, enabling the encapsulation of various cargo.

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Directed Assembly Elastin-like Proteins Supramolecular Structures Cargo Encapsulation In Vitro Amphiphilic Physicochemical Properties Vesicles Fibers Coacervates Protein Membrane-based Compartments Phase Separation Behavior Fluorescent Cargo Molecules Expression Culture F20E20-mEGFP F20E20-mCherry Inoculate OD600 IPTG Unnatural Amino Acid E.coli Pre-culture LB Medium Kanamycin Chloramphenicol
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