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使用激光切割模具实现简单的无光刻单细胞微模式

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

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使用激光切割模具实现简单的无光刻单细胞微模式

DOI:

10.3791/60888-v

•

08:59 min

•

April 03, 2020

•

Soah Lee*^1,2,3, Huaxiao Yang*^1,2,3, Caressa Chen*^1,2,3, Sneha Venkatraman^2,3, Adrija Darsha^2,3, Sean M. Wu^2,3, Joseph C. Wu^2,3, Timon Seeger^2,3,4,5

¹Stanford Cardiovascular Institute,Stanford University School of Medicine, ²Department of Medicine, Division of Cardiovascular Medicine,Stanford University, ³Institute for Stem Cell Biology and Regenerative Medicine,Stanford University, ⁴Department of Medicine III,University Hospital Heidelberg, ⁵German Centre for Cardiovascular Research (DZHK),Partner Site Heidelberg/Mannheim

Capítulos

00:04Introduction
00:55Solution Preparation
01:48Hydrogel Fabrication
03:43Protein Conjugation
06:24Results: Micropatterning of Cells
07:52Conclusion

Summary

Tadução automática

该协议引入了一种无光刻图的微模式方法，该方法对于生物工程背景有限的人来说简单且易于使用。该方法利用定制的激光切割模具，以感兴趣的形状进行微模式细胞外基质蛋白，以调节细胞形态。微模式程序使用诱导多能干细胞衍生心肌细胞进行演示。

Tags

Single Cell Micropatterning Laser-cut Stencils Lithography-free Polyacrylamide Hydrogel Basement Membrane Matrix UV Cross-linking Cell Shape Mechanotransduction High-throughput Drug Screening Disease Modeling

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