This protocol introduces a lithography-free micropatterning method that is simple and accessible to those with a limited bioengineering background. This method utilizes customized laser-cut stencils to micropattern extracellular matrix proteins in a shape of interest for modulating cell morphologies. The procedure for micropatterning is demonstrated using induced pluripotent stem cell derived cardiomyocytes.
Lee, S., Yang, H., Chen, C., Venkatraman, S., Darsha, A., Wu, S. M., Wu, J. C., Seeger, T. Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils. J. Vis. Exp. (158), e60888, doi:10.3791/60888 (2020).