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Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils
JoVE Journal
Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

7,297 Views

08:59 min

April 03, 2020

DOI:

08:59 min
April 03, 2020

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Summary

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This protocol introduces a lithography-free micropatterning method that is simple and accessible to those with a limited bioengineering background. This method utilizes customized laser-cut stencils to micropattern extracellular matrix proteins in a shape of interest for modulating cell morphologies. The procedure for micropatterning is demonstrated using induced pluripotent stem cell derived cardiomyocytes.

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