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Biologia do Desenvolvimento

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使用全载免疫作用化学和三维重建对咽弓动脉的可视化与分析

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction

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使用全载免疫作用化学和三维重建对咽弓动脉的可视化与分析

DOI:

10.3791/60797-v

•

10:02 min

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March 31, 2020

•

AnnJosette Ramirez², Sophie Astrof

¹Department of Cell Biology and Molecular Medicine, New Jersey Medical School,Rutgers Biomedical and Health Sciences, ²Multidisciplinary PhD Program in Biomedical Sciences: Cell Biology, Neuroscience and Physiology Track, New Jersey Medical School,Rutgers Biomedical and Health Sciences

Capítulos

00:04Introduction
00:55Embryo Staining and Embedding
03:03Methanol Dehydration and Tissue Clearing
04:04Embryo Mounting
04:40Third Pharyngeal Arch (PA) Surfacing
05:57Surfaced Structure Masking
07:01Endothelial Cell Quantification
08:19Results: Representative PA and PAA Surfacing and EC Quantification
09:28Conclusion

Summary

Tadução automática

在这里，我们描述了一种协议，使用全孔免疫荧光、组织清除、共聚焦显微镜和3D重建来可视化和分析小鼠胚胎的咽弓动脉3、4和6。

Tags

Keywords: Visualization Analysis Pharyngeal Arch Arteries Whole-mount Immunohistochemistry 3D Reconstruction Endothelial Cell Quantification Aortic Arch Arteries Congenital Heart Disease Vessel Formation Remodeling Light-sheet Microscopy Mouse Embryo Fixation Permeabilization Blocking Buffer Primary Antibody Secondary Antibody Plastic Paraffin Molds

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