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Imunologia e Infecção
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Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

DOI:
10.3791/60743-v

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08:23 min

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March 02, 2020

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, , ,
1Centre for Superbug Solutions, Institute for Molecular Biosciences,The University of Queensland

Capítulos

  • 00:05Introduction
  • 00:33Fluorescent Antibiotic Synthesis
  • 02:16Antimicrobial Activity Evaluation
  • 04:19Probe Accumulation Analysis
  • 07:00Results: Representative Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
  • 07:48Conclusion

Summary

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Fluorescently tagged antibiotics are powerful tools that can be used to study multiple aspects of antimicrobial resistance. This article describes the preparation of fluorescently tagged antibiotics and their application to studying antibiotic resistance in bacteria. Probes can be used to study mechanisms of bacterial resistance (e.g., efflux) by spectrophotometry, flow cytometry, and microscopy.

Tags

Fluorescent AntibioticsClick ChemistryAzideAlkyneCopper SulfateAscorbic AcidLiquid Chromatography Mass SpectrometryLCMSAntimicrobial ActivityBacterial StrainsLB AgarCAMHBOptical DensityDimethyl Sulfoxide

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