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Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
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Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
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March 02, 2020
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Fluorescently tagged antibiotics are powerful tools that can be used to study multiple aspects of antimicrobial resistance. This article describes the preparation of fluorescently tagged antibiotics and their application to studying antibiotic resistance in bacteria. Probes can be used to study mechanisms of bacterial resistance (e.g., efflux) by spectrophotometry, flow cytometry, and microscopy.
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Stone, M. R. L., Phetsang, W., Cooper, M. A., Blaskovich, M. A. T. Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes. J. Vis. Exp. (157), e60743, doi:10.3791/60743 (2020).
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