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Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
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JoVE Journal Genética
Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

DOI:
10.3791/60697-v

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10:19 min

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February 03, 2020

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, , , , , ,
1Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences,Shanghai Normal University, 2College of Agriculture,Yangtze University, 3Laboratory of Molecular Genetics, China National Tobacco Corporation,Guizhou Institute of Tobacco Science

Capítulos

  • 00:04Introduction
  • 00:26Preparation of N. benthamiana 16c Plants
  • 02:08Preparation of Agrobacterium Culture for Infiltration
  • 03:44Co-infiltration N. benthamiana Leaves
  • 04:26GFP Imaging Analysis
  • 04:47Northern Blot Analysis of GFP mRNA Levels in Infiltrated Leaves
  • 08:08Results: Infiltrated N. benthamiana 16c Leaves
  • 09:24Conclusion

Summary

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Here, we present a modified screening method that can be extensively used to quickly screen RNA silencing suppressors in plant pathogens.

Tags

RNA Silencing SuppressorsPlant PathogensScreening AssayNicotiana BenthamianaSystemic RNA SilencingLocal RNA SilencingAgrobacteriumLB MediumAntibioticsMESAcetosyringone

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