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从高致病性H5N1病毒和禽流感病毒中生产含有包络糖蛋白的高蒂特感染性流感伪型颗粒
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses
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从高致病性H5N1病毒和禽流感病毒中生产含有包络糖蛋白的高蒂特感染性流感伪型颗粒

DOI:
10.3791/60663-v

•

08:10 min

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January 15, 2020

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, , , , , ,
1Central Laboratory,Hang Zhou Red Cross Hospital, 2Clinical Laboratory,Yuhang District Maternity and Child Health Care Hospital

Capítulos

  • 00:04Título
  • 00:39Four-plasmid Cotransfection Mediated by Lipofection
  • 02:15Susceptible Cell Seeding and Pseudotyped Viral Particle Quantification
  • 04:01Infectivity Assay
  • 05:34Results: Infectivity Assay of Pseudotyped Particles
  • 06:58Conclusion

Summary

Tadução automática

Tadução automática

该协议描述了一个实验过程，以产生高蒂特传染性病毒伪型颗粒（pp），包络糖蛋白从两个流感A毒株，以及如何确定其传染性。该协议具有很强的适应性，可开发具有不同包络糖蛋白的任何其他类型的包络病毒的pps。

Tags

InfluenzaPseudotyped ParticlesViral Envelope GlycoproteinsH5N1H7N9BSL-2TransfectionQRT-PCRVirus ReassortmentCell CultureIncubationHarvestQuantificationBenzonase Nuclease

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