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用于基因表达分析的小鼠胚胎软骨和骨骼的激光捕获微分
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
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用于基因表达分析的小鼠胚胎软骨和骨骼的激光捕获微分

6,868 Views

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09:20 min

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December 18, 2019

DOI:

10.3791/60503-v

DOI:
10.3791/60503-v

•

09:20 min
December 18, 2019

•

3 Views
, , , ,
1Department of Genetics and Genomic Sciences,Icahn School of Medicine at Mount Sinai, 2Icahn Institute for Data Science and Genomic Technology,Icahn School of Medicine at Mount Sinai

Summary

Automatically generated

该协议描述了激光捕获微解剖，用于从小鼠胚胎的新鲜冷冻部分分离软骨和骨骼。软骨和骨骼可以通过紫罗兰染色快速可视化，并精确收集，产生高质量的RNA进行转录分析。

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Cite this Article

Wu, M., Kriti, D., van Bakel, H., Jabs, E. W., Holmes, G. Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis. J. Vis. Exp. (154), e60503, doi:10.3791/60503 (2019).

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