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Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis
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Capítulos
- 00:04Título
- 00:59Silicon Nitride (Si3N4) Membrane Support Preparation
- 02:14Cell Seeding
- 03:04Plunge-Freezing
- 04:46Plunge-Frozen Cell Freeze-Drying
- 06:05Results: Representative X-Ray Fluorescence Imaging of Frozen Hydrated and Freeze-Dried Cells
- 07:28Conclusion
Presented here is a protocol for cell culture on silicon nitride membranes and plunge-freezing prior to X-ray fluorescence imaging with a synchrotron cryogenic X-ray nanoprobe. When only room temperature nano-analysis is provided, the frozen samples can be further freeze-dried. These are critical steps to obtain information on the intracellular elemental composition.
