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変動アッセイを用いて微生物突然変異率を測定する
JoVE Journal
Genética
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JoVE Journal Genética
Measuring Microbial Mutation Rates with the Fluctuation Assay
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変動アッセイを用いて微生物突然変異率を測定する

22,095 Views

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07:44 min

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November 28, 2019

DOI:

10.3791/60406-v

DOI:
10.3791/60406-v

•

07:44 min
November 28, 2019

•

12 Views
, , , , ,
1School of Biological Sciences,The University of Manchester, 2School of Science,University of Waikato, 3School of Natural Sciences,The University of Manchester, 4Department of Mathematics,Université du Québec à Montréal

Tags

Microbial Mutation RatesFluctuation AssayAntibiotic ResistanceAntimicrobial ResistanceMonocultureCo-cultureE. Coli K12Davis Minimal MediumGlucoseOptical DensityInoculumDeep Well PlateIncubationEvaporation

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Krašovec, R., Richards, H., Gomez, G., Gifford, D. R., Mazoyer, A., Knight, C. G. Measuring Microbial Mutation Rates with the Fluctuation Assay. J. Vis. Exp. (153), e60406, doi:10.3791/60406 (2019).

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