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Measuring Microbial Mutation Rates with the Fluctuation Assay
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Measuring Microbial Mutation Rates with the Fluctuation Assay

Measuring Microbial Mutation Rates with the Fluctuation Assay

22,354 Views

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07:44 min

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November 28, 2019

DOI:

10.3791/60406-v

DOI:
10.3791/60406-v

•

07:44 min
November 28, 2019

•

36 Views
, , , , ,
1School of Biological Sciences,The University of Manchester, 2School of Science,University of Waikato, 3School of Natural Sciences,The University of Manchester, 4Department of Mathematics,Université du Québec à Montréal

Summary

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Here, a protocol is presented to perform a fluctuation assay and estimate microbial mutation rate using phenotypic markers. This protocol will enable researchers to assay mutations in diverse microbes and environments, determining how genotype and ecological context affect spontaneous mutation rates.

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Krašovec, R., Richards, H., Gomez, G., Gifford, D. R., Mazoyer, A., Knight, C. G. Measuring Microbial Mutation Rates with the Fluctuation Assay. J. Vis. Exp. (153), e60406, doi:10.3791/60406 (2019).

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