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Purkinje-cellernes overlevelse i Udsnitskulturer af Organotypiske cerebellar
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Neurociência
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JoVE Journal Neurociência
Purkinje Cell Survival in Organotypic Cerebellar Slice Cultures

Purkinje-cellernes overlevelse i Udsnitskulturer af Organotypiske cerebellar

DOI:
10.3791/60353-v

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06:31 min

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December 18, 2019

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1Department of Physiology,Northwestern University, Feinberg School of Medicine

Capítulos

  • 00:04Título
  • 00:58Organotypic Cerebellar Slice Culture Preparation
  • 01:40Organotypic Cerebellar Slice Culture Dissection
  • 03:32Immunofluorescence
  • 05:24Results: Representative Purkinje Cell Survival in Organotypic Cerebellar Slice Culture Following High Potassium Chloride (KCl) Treatment
  • 05:54Conclusion

Summary

Tadução automática

Tadução automática

Organotypiske skive kulturer er et kraftfuldt værktøj til at studere neuroudviklingsmæssige eller degenerative/regenerative processer. Her beskriver vi en protokol, der modeller den neuroudviklingsmæssige død af Purkinje celler i mus cerebellar skive kulturer. Denne metode kan gavne forskning i neuroprotektive Drug Discovery.

Tags

Keywords: Purkinje CellOrganotypic Slice CultureCerebellar SlicesNeuroprotectionNeurodegenerative DiseasesCell-cell ConnectionsIn Vivo ConditionsPrimary Cell CultureCerebellumParasagittal Sections

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