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细菌细胞的三维成像，实现精确的细胞表现和精确的蛋白质定位
JoVE Journal
Imunologia e Infecção
This content is Free Access.
JoVE Journal Imunologia e Infecção
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
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细菌细胞的三维成像，实现精确的细胞表现和精确的蛋白质定位

DOI:
10.3791/60350-v

•

06:33 min

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October 29, 2019

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, ,
1Department of Microbiology and Molecular Genetics,Oklahoma State University, 2Department of Molecular Biology and Lewis-Sigler Institute of Integrative Genomics,Princeton University

Capítulos

  • 00:04Título
  • 00:22Slide Preparation
  • 01:41Imaging
  • 03:05Cell Reconstruction
  • 05:10Results: Representative Assessment of Protein Localization to Specific Cellular Geometries
  • 06:00Conclusion

Summary

Tadução automática

Tadução automática

该协议解释了如何准备和安装细菌样本进行活的三维成像，以及如何从这些图像中重建大肠杆菌的三维形状。

Tags

3D ImagingBacterial CellsCellular RepresentationProtein LocalizationMicroscopyAgarose PadsE. ColiZ-stackFluorescence Imaging

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