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Isolering av regionspecifika Microglia från en vuxen mus hjärnhalva halvklotet för djup Single-cell RNA sekvensering

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

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Isolering av regionspecifika Microglia från en vuxen mus hjärnhalva halvklotet för djup Single-cell RNA sekvensering

10,318 Views

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09:49 min

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December 03, 2019

DOI:

10.3791/60347-v

DOI:

10.3791/60347-v

•

09:49 min

December 03, 2019

•

3 Views

Lu Zhou, Qingyun Li

¹Department of Neurobiology,Stanford University School of Medicine

Tags

Microglia Single-cell RNA Sequencing Brain Regions Cell Isolation Cell Separation Dounce Homogenizer Magnetic Separation RNA Extraction Library Preparation Deep Sequencing

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