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Neuroscience

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JoVE Journal Neuroscience
Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
 

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Article DOI: 10.3791/60347-v • 09:49 min • December 3rd, 2019
December 3rd, 2019 •
1, 1
1Department of Neurobiology, Stanford University School of Medicine

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We provide a protocol for isolation of microglia from different dissected regions of an adult mouse brain hemisphere, followed by semi-automated library preparation for deep single-cell RNA sequencing of full-length transcriptomes. This method will help to elucidate functional heterogeneity of microglia in health and disease.

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Isolation Region-specific Microglia Adult Mouse Brain Hemisphere Deep Single-cell RNA Sequencing Unbiased Capturization Microglia Gene Expression Molecular Heterogeneity Healthy And Diseased Brain Detailed Procedure Rapid Isolation Brain Regions Single-cell RNA Libraries Deep Sequencing Buffers And Solutions Euthanizing And Decapitating The Mouse Small Scissors Sagittal Suture Lambdoidal Suture Coronal Suture Forceps Parietal Bone Interparietal Bone Dissection Petri Dish Medium A Pre-chilled Blade Cerebellum Cortical Lobe Brain Stem Hippocampus Striatum Collection Petri Dish
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