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全斑马鱼胚胎的延长时移共聚焦显微镜的分层安装方法
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos
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全斑马鱼胚胎的延长时移共聚焦显微镜的分层安装方法

DOI:
10.3791/60321-v

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08:55 min

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January 14, 2020

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, , , , ,
1Computational Biomedicine Lab, Texas Institute of Measurement Evaluation and Statistics,University of Houston, 2Institute of Biosciences and Technology,Texas A&M Health Science Center, 3Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling,University of Houston, 4Department of Biosciences and Nutrition,Novum, Karolinska Institutet, 5Department of Intelligent Systems Engineering,Indiana University

Capítulos

  • 00:04Título
  • 00:41Fish Husbandry and Preparation of Solutions and Embryos
  • 01:44Mounting in Agarose and Optimization of Agarose Solution
  • 03:38Time-lapse Imaging
  • 06:07Results: Extended Time-lapse Imaging of Zebrafish Embryos
  • 07:54Conclusion

Summary

Tadução automática

Tadução automática

本文介绍了一种将脆弱的斑马鱼胚胎用于延长延时共聚焦显微镜的方法。这种低成本的方法很容易使用常规玻璃底显微镜盘在任何倒置显微镜上成像。安装以不同浓度的甘蔗层进行。

Tags

Time-lapse ImagingZebrafish EmbryosMounting MethodConfocal MicroscopyAgaroseTricaineDechorionationGlass Bottom Dish

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