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Avbildning av intracellulära ATP i Organotypic vävnad skivor av musen hjärnan med hjälp av bandet-baserade sensorn ATeam 1,03Yemk
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK
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Avbildning av intracellulära ATP i Organotypic vävnad skivor av musen hjärnan med hjälp av bandet-baserade sensorn ATeam 1,03Yemk

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11:20 min

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December 19, 2019

DOI:

10.3791/60294-v

DOI:
10.3791/60294-v

•

11:20 min
December 19, 2019

•

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, , , , ,
1Institute of Neurobiology,Heinrich Heine University Düsseldorf, 2Department of Neurology,Düsseldorf University Hospital

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Intracellular ATPMouse BrainFRET-based SensorATeam1.03YEMKFluorescent ImagingATP MeasurementsNeuronal ATPAstrocytic ATPEnergy MetabolismIschemic StrokeHippocampal FormationSterile ConditionsPetri DishesHanks’ Salt Solution

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Lerchundi, R., Kafitz, K. W., Färfers, M., Beyer, F., Huang, N., Rose, C. R. Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK. J. Vis. Exp. (154), e60294, doi:10.3791/60294 (2019).

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