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从果蝇雄性附件腺的二级细胞中分离RNA的FACS协议

JoVE Journal
Genética

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JoVE Journal Genética

A FACS-based Protocol to Isolate RNA from the Secondary Cells of Drosophila Male Accessory Glands

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从果蝇雄性附件腺的二级细胞中分离RNA的FACS协议

DOI:

10.3791/60218-v

•

06:54 min

•

September 05, 2019

•

Clément Immarigeon, François Karch, Robert K. Maeda

¹Department of Genetics and Evolution,University of Geneva

Capítulos

00:05Título
01:03Accessory Gland Dissection
02:50Tissue Digestion and Mechanical Cell Dissociation
04:08Results: Representative Secondary Cell (SC) RNA Isolation and Analysis
05:55Conclusion

Summary

Tadução automática

在这里，我们提出一个协议，从果蝇雄性附属腺体（辅助细胞）分离和排序特定细胞群，用于RNA测序和RT-qPCR。细胞分离是通过FACS纯化GFP表达的二次细胞后，需要解剖，蛋白酶消化和机械分散多步骤解散过程。

Tags

Keywords: FACS Drosophila Male Accessory Glands Secondary Cells Transcriptomic Analysis RNA Extraction Cell Sorting

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