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A FACS-based Protocol to Isolate RNA from the Secondary Cells of Drosophila Male Accessory Glands
JoVE Journal
Genetica
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JoVE Journal Genetica
A FACS-based Protocol to Isolate RNA from the Secondary Cells of Drosophila Male Accessory Glands

A FACS-based Protocol to Isolate RNA from the Secondary Cells of Drosophila Male Accessory Glands

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06:54 min

September 05, 2019

DOI:

06:54 min
September 05, 2019

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Summary

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Here, we present a protocol to dissociate and sort a specific cell population from the Drosophila male accessory glands (secondary cells) for RNA sequencing and RT-qPCR. Cell isolation is accomplished through FACS purification of GFP-expressing secondary cells after a multistep-dissociation process requiring dissection, proteases digestion and mechanical dispersion.

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