We describe an in vivo method to investigate the functional loss of a specific gene in satellite cells by using a combination of cardiotoxin mediated injury of skeletal muscle and the injection of a self-delivering siRNA.
Ahrens, H. E., Henze, H., Schüler, S. C., Schmidt, M., Hüttner, S. S., von Maltzahn, J. Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo. J. Vis. Exp. (151), e60194, doi:10.3791/60194 (2019).