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JoVE Journal Developmental Biology
An Ectopic Chemokine Expression Model for Testing Macrophage Recruitment In Vivo
 

An Ectopic Chemokine Expression Model for Testing Macrophage Recruitment In Vivo

Article DOI: 10.3791/60161-v • 10:02 min • September 25th, 2019
September 25th, 2019 •
1, 1, 2
1Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, 2Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology

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To test the effect of a chemokine on macrophage recruitment in vivo, the whole mount in situ hybridization was used to detect the ectopic expression of the chemokine, and immunostaining was used to label macrophages. Live imaging was used for real-time observation of macrophage migration.

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Ectopic Chemokine Expression Model Macrophage Recruitment In Vivo Experimental Models Cell Chemotaxis In Vitro Experiments Chemoattraction Ability Zebra Fish TGFABP-10A Interleukin-34 Transgenic Constructs Inject FABP-10A Interleukin-34 Constructs Tg(mpeg1:GFP)transgenic Fish Embryos Fix With 4% Paraformaldehyde Wash With PBST Dehydrate With Methanol Probe Hybridization Proteinase K Digestion
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