Journal
/
/
Immunofluorescence Labelling of Human and Murine Neutrophil Extracellular Traps in Paraffin-Embedded Tissue
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
Immunofluorescence Labelling of Human and Murine Neutrophil Extracellular Traps in Paraffin-Embedded Tissue

Immunofluorescence Labelling of Human and Murine Neutrophil Extracellular Traps in Paraffin-Embedded Tissue

DOI:

07:17 min

September 10, 2019

,

Capítulos

  • 00:04Título
  • 00:52Rehydration, Heat-induced Epitope Retrieval, Staining, Mounting, and Microscopic Analysis
  • 03:57Results: Immunofluorescence Labelling of Neutrophil Extracellular Traps in Paraffin-Embedded Tissue
  • 06:16Conclusion

Summary

Tadução automática

Neutrophil extracellular traps (NETs) are three-dimensional structures generated by stimulated neutrophil granulocytes. It has become clear in recent years that NETs are involved in a wide variety of diseases. Detection of NETs in tissue may have diagnostic relevance, so standardized protocols for labelling NET components are required.

Vídeos Relacionados

Read Article