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SUMO-Binding Entities (SUBEs) als Werkzeuge zur Anreicherung, Isolierung, Identifizierung und Charakterisierung des SUMO-Proteoms bei Leberkrebs
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
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SUMO-Binding Entities (SUBEs) als Werkzeuge zur Anreicherung, Isolierung, Identifizierung und Charakterisierung des SUMO-Proteoms bei Leberkrebs

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08:29 min

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November 01, 2019

DOI:

10.3791/60098-v

DOI:
10.3791/60098-v

•

08:29 min
November 01, 2019

•

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1Liver Disease and Liver Metabolism Lab,CIC bioGUNE, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), 2Proteomics Platforms,CIC bioGUNE, ProteoRED-ISCIII, CIBERehd, 3Advanced Technology Institute in Life Sciences (ITAV)-CNRS-IPBS,UbiCARE

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SUMO-binding EntitiesSUBEsSUMO ProteomeLiver CancerSUMOylationProtein IsolationProtein CharacterizationProteaseTandem RepeatsSIMsTherapeutic ApproachHuman Hepatoma CellsCell LysisTrypsinization

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Lopitz-Otsoa, F., Delgado, T. C., Lachiondo-Ortega, S., Azkargorta, M., Elortza, F., Rodríguez, M. S., Martínez-Chantar, M. L. SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer. J. Vis. Exp. (153), e60098, doi:10.3791/60098 (2019).

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