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使用由内皮结肠形成细胞和间质干细胞形成的共培养球体的三维血管生成测定系统
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells
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使用由内皮结肠形成细胞和间质干细胞形成的共培养球体的三维血管生成测定系统

DOI:
10.3791/60032-v

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09:24 min

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September 18, 2019

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, , , , , , ,
1College of Pharmacy, Duksung Innovative Drug Center,Duksung Women’s University, 2Korea Drug Development Platform using Radio-isotope,Korea Institute of Radiological & Medical Sciences

Capítulos

  • 00:04Título
  • 01:06Endothelial Colony Forming Cell (ECFC) and Mesenchymal Stem Cell (MSC) Suspension Preparation and Fluorescent Dye Labeling
  • 03:20ECFC, MSC, or ECFC+MSC Spheroid Generation
  • 04:12Neutralized Collagen Gel Spheroid Embedding and Spheroid Sprouting Quantification
  • 06:29Results: Representative Sprout Formation from ECFC-only, MSC-only, and ECFC+MSC Spheroids
  • 08:44Conclusion

Summary

Tadução automática

Tadução automática

三维共培养球形血管生成测定系统设计用于模拟生理血管生成。共培养球体由两个人类血管细胞前体ECFC和MSCs组成，并嵌入胶原蛋白凝胶中。新系统对血管生成调节器的评估是有效的，为体内研究提供了更多的相关信息。

Tags

3D Co-culture SpheroidAngiogenesisEndothelial Colony Forming Cells (ECFCs)Mesenchymal Stem Cells (MSCs)Vascular Progenitor CellsCell InteractionsSproutingTubular MaturationPersonalized TreatmentsAbnormal Angiogenesis-related DiseasesCancerDrug DevelopmentTrypsin-EDTASingle Cell SuspensionPKH67PKH26Fluorescent DyeSpheroid Generation

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