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Karakterisering av membran transportörer genom heterologt uttryck i E. coli och produktion av membran blåsor
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Bioquímica
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JoVE Journal Bioquímica
Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
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Karakterisering av membran transportörer genom heterologt uttryck i E. coli och produktion av membran blåsor

8,967 Views

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13:16 min

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December 31, 2019

DOI:

10.3791/60009-v

DOI:
10.3791/60009-v

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13:16 min
December 31, 2019

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1 Views
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1Department of Biological Sciences,Bowling Green State University

Tags

Membrane TransportersHeterologous ExpressionE. ColiMembrane VesiclesProtein GradientsSubstrate TransportAffinityCompetition AssaysSubstrate ActivationFrench PressUltracentrifugation

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Ariyaratne, M., Ge, L., Morris, P. F. Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles. J. Vis. Exp. (154), e60009, doi:10.3791/60009 (2019).

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