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Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles

Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles

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13:16 min

December 31, 2019

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13:16 min
December 31, 2019

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We describe a method for the characterization of proton-driven membrane transporters in membrane vesicle preparations produced by heterologous expression in E. coli and lysis of cells using a French press.

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