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大肠杆菌中异构表达对膜迁移者的特性与膜囊泡的产生

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles

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大肠杆菌中异构表达对膜迁移者的特性与膜囊泡的产生

DOI:

10.3791/60009-v

•

13:16 min

•

December 31, 2019

•

Menaka Ariyaratne, Lingxiao Ge, Paul F. Morris

¹Department of Biological Sciences,Bowling Green State University

Capítulos

00:05Título
01:18Generation of Inside-out Membrane Vesicles
04:21Western Blot and Orientation of Transporter Assay
06:46Transport Assay
08:44Results: Characterization of Membrane Transporters
11:05Conclusion

Summary

Tadução automática

我们描述了一种在大肠杆菌中的异质表达和利用法国印刷机对细胞进行分理的膜囊泡制剂中质子驱动膜输送剂的表征方法。

Tags

Keywords: Membrane Transporters Heterologous Expression E. Coli Membrane Vesicles Protein Gradients Substrate Transport Affinity Competition Assays Substrate Activation French Press Ultracentrifugation

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