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从多能干细胞中无血清自由生产三维人类肝球

JoVE Journal
Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells

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从多能干细胞中无血清自由生产三维人类肝球

DOI:

10.3791/59965-v

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06:57 min

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July 20, 2019

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Balta Lucendo-Villarin, Hassan Rashidi², Sharmin Alhaque³, Lena Fischer⁴, Jose Meseguer-Ripolles, Yu Wang, Cliona O’Farrelly, Michael Themis, David C. Hay

¹MRC Centre for Regenerative Medicine,University of Edinburgh, ²UCL Great Ormond Street Institute of Child Health,University College London, ³Division of Biosciences, Department of Life Sciences, College of Health and Life Sciences,Brunel University London, ⁴School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute,Trinity College Dublin

Capítulos

00:04Título
00:31Preparation of Agarose Microplate Molds
01:12Seeding Human Pluripotent Stem Cells into Agarose Microwell Plates
03:07Differentiating hPSCs to 3D Hepatospheres
05:28Results: Hepatosphere Structure and Function
06:16Conclusion

Summary

Tadução automática

该协议描述了一种使用定义的培养系统和细胞自组装从人类多能干细胞中产生肝球的方法。该协议可重复在多个细胞系中,具有成本效益,并允许生产稳定的人类肝球用于生物医学应用。

Tags

Keywords: 3D Hepatospheres Pluripotent Stem Cells Serum-free Agarose Molds Cell Suspension Rock Inhibitor Cell Culture

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