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极快速和特殊的代谢标签的RNA在Vivo与4-Thiouracil （Ers4tU）

JoVE Journal
Bioquímica

This content is Free Access.

JoVE Journal Bioquímica

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)

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极快速和特殊的代谢标签的RNA在Vivo与4-Thiouracil （Ers4tU）

DOI:

10.3791/59952-v

•

00:11 min

•

August 22, 2019

•

J. David Barrass, Jean D. Beggs

¹Wellcome Centre for Cell Biology, School of Biological Sciences,University of Edinburgh

Capítulos

00:04Título
00:38Growth and Thio-labeling
02:55Preparation of Total RNA
04:38Biotinylation
05:58Purification of the Newly Synthesized RNA
09:29Results: In Vivo Labelling of RNA with 4-Thiouracil
10:44Conclusion

Summary

Tadução automática

使用硫化尿素来敏感和专门地从酵母糖母体中纯化新转录的RNA。

Tags

Keywords: 4-Thiouracil RNA Labeling In Vivo Pulse-chase Experiments Protein Depletion Thiolation Yeast S. Pombe Metabolic Labeling RNA Extraction AE Buffer Phenol Extraction

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