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Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)
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Bioquímica
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JoVE Revista Bioquímica
Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)

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00:11 min

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August 22, 2019

DOI:

10.3791/59952-v

DOI:
10.3791/59952-v

•

00:11 min
August 22, 2019

•

1 Views
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1Wellcome Centre for Cell Biology, School of Biological Sciences,University of Edinburgh

Tags

4-ThiouracilRNA LabelingIn VivoPulse-chase ExperimentsProtein DepletionThiolationYeastS. PombeMetabolic LabelingRNA ExtractionAE BufferPhenol Extraction

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Barrass, J. D., Beggs, J. D. Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU). J. Vis. Exp. (150), e59952, doi:10.3791/59952 (2019).

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