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荧光标记细菌作为追踪器揭示水生生态系统中有机碳流动的新途径
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Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems
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荧光标记细菌作为追踪器揭示水生生态系统中有机碳流动的新途径

DOI:
10.3791/59903-v

•

09:35 min

•

September 13, 2019

•

,
1Department of Aquatic Microbial Ecology, Institute of Hydrobiology,Biology Centre CAS

Capítulos

  • 00:04Título
  • 01:11Sample Collection and Fixation
  • 01:44Sample Filtration and Enumeration of Bacterial Numbers on the Filters
  • 02:45Determining Protistan Abundance
  • 03:28Estimating Ciliate Grazing Rates
  • 04:33Results: Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems
  • 08:07Conclusion

Summary

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这里介绍的是一种基于单细胞的表氟显微镜技术的协议，用于以高精度和分类分辨率量化水生捕食真核生物的放牧率。

Tags

Fluorescently Labeled BacteriaOrganic Carbon FlowAquatic EcosystemsMicrobial Food WebsPredator-prey InteractionsGrazing RatesTrophic InteractionsNutrient TransferSingle-cell AnalysisMicroscopyEpifluorescenceAquatic Microbial Communities

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