Journal
/
环境科学
/
Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems
JoVE 杂志
环境科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 环境科学
Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems

Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems

DOI:
10.3791/59903-v

•

09:35 min

•

September 13, 2019

•

,
1Department of Aquatic Microbial Ecology, Institute of Hydrobiology,Biology Centre CAS

Chapters

  • 00:04Title
  • 01:11Sample Collection and Fixation
  • 01:44Sample Filtration and Enumeration of Bacterial Numbers on the Filters
  • 02:45Determining Protistan Abundance
  • 03:28Estimating Ciliate Grazing Rates
  • 04:33Results: Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems
  • 08:07Conclusion

Summary

自动翻译

自动翻译

Presented here is a protocol for a single-cell, epifluorescence microscopy-based technique to quantify grazing rates in aquatic predatory eukaryotes with high precision and taxonomic resolution.

Tags

Keywords: Fluorescently Labeled BacteriaOrganic Carbon FlowAquatic EcosystemsMicrobial Food WebsPredator-prey InteractionsGrazing RatesTrophic InteractionsNutrient TransferSingle-cell AnalysisMicroscopyEpifluorescenceAquatic Microbial Communities

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/59903?article_type=v

Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals
check_url/cn/59903?article_type=v

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
check_url/cn/59903?article_type=v

Alternative Method of Removing Otoliths from Sturgeon
check_url/cn/59903?article_type=v

Assessment of Labile Organic Carbon in Soil Using Sequential Fumigation Incubation Procedures
check_url/cn/59903?article_type=v

Understanding Dissolved Organic Matter Biogeochemistry Through In Situ Nutrient Manipulations in Stream Ecosystems
check_url/cn/59903?article_type=v

A Flow-through Exposure System for Evaluating Suspended Sediments Effects on Aquatic Life
check_url/cn/59903?article_type=v

Production and Measurement of Organic Particulate Matter in a Flow Tube Reactor
check_url/cn/59903?article_type=v

Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions
check_url/cn/59903?article_type=v

Characterization of Aquatic Biofilms with Flow Cytometry
check_url/cn/59903?article_type=v

Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine

Read Article