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Assessing Mineral Availability in Fish Feeds using Complementary Methods Demonstrated with the Example of Zinc in Atlantic Salmon
JoVE Journal
Biologia
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JoVE Journal Biologia
Assessing Mineral Availability in Fish Feeds using Complementary Methods Demonstrated with the Example of Zinc in Atlantic Salmon

Assessing Mineral Availability in Fish Feeds using Complementary Methods Demonstrated with the Example of Zinc in Atlantic Salmon

DOI:
10.3791/59862-v

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04:54 min

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October 29, 2021

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, , , , , , , , ,
1Institute of Marine Research, 2Department of Biological Sciences,University of Bergen, 3National Food Institute,Technical University of Denmark, 4Metal metabolism group, Nutritional Sciences Division,King’s College London

Capítulos

  • 00:04Introduction
  • 00:31In Vitro Solubility of Supplemented Zn in Atlantic Salmon
  • 01:33Cell Harvest and Preparing for Exposure Experiments
  • 02:49Apparent Availability of Dietary Zn in Atlantic Salmon (Salmo salar)
  • 03:13Results: Mineral Availability in Fish Feeds
  • 04:23Conclusion

Summary

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This article explains in detail a systematic approach to assess micro-mineral availability in Atlantic salmon. The methodology includes tools and models with increasing biological complexity: (1) chemical speciation analysis, (2) in vitro solubility, (3) uptake studies in cell lines, and (4) in vivo fish studies. 

Tags

Mineral AvailabilityFish FeedZincAtlantic SalmonRadionuclideSolubilityAmino AcidsIntestinal Luminal BufferCell LineFecesSize-exclusion ChromatographyInductively-coupled Plasma Mass Spectrometry

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