This article explains in detail a systematic approach to assess micro-mineral availability in Atlantic salmon. The methodology includes tools and models with increasing biological complexity: (1) chemical speciation analysis, (2) in vitro solubility, (3) uptake studies in cell lines, and (4) in vivo fish studies.
Silva, M. S., Stewart, T., Amlund, H., Sloth, J. J., Araujo, P., Lock, E., Hogstrand, C., Ørnsrud, R., Waagbø, R., Prabhu, A. J. Assessing Mineral Availability in Fish Feeds using Complementary Methods Demonstrated with the Example of Zinc in Atlantic Salmon. J. Vis. Exp. (176), e59862, doi:10.3791/59862 (2021).