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使用新激酶蛋白的强生化方法在分子水平上进行表征
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Bioquímica
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JoVE Journal Bioquímica
Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
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使用新激酶蛋白的强生化方法在分子水平上进行表征

5,999 Views

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11:23 min

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June 30, 2019

DOI:

10.3791/59820-v

DOI:
10.3791/59820-v

•

11:23 min
June 30, 2019

•

4 Views
, , ,
1Centre de Biophysique Moléculaire, CNRS, UPR 4301,University of Orléans and INSERM

Summary

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我们使用强大的生化方法对一种新的激酶蛋白进行了定性:用特殊抗体对不同细胞系和组织进行西带体分析,通过共免疫沉淀实验相互作用,由西方检测到的激酶活性使用磷特异性抗体和由 β+32P+ ATP 标记的斑点。

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Cite this Article

Vallée, B., Doudeau, M., Godin, F., Bénédetti, H. Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein. J. Vis. Exp. (148), e59820, doi:10.3791/59820 (2019).

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